abstract：:We show how to speed up sequence alignment algorithms of the type introduced by Needleman and Wunsch (and generalized by Sellers and others). Faster alignment algorithms have been introduced, but always at the cost of possibly getting sub-optimal alignments. Our modification results in the optimal alignment still bein...

journal_title：Nucleic acids research

authors： Fickett JW

更新日期：1984-01-11 00:00:00

abstract：:We describe an interactive computer program (ANALYSEQ) that is used from a simple graphics terminal. The main purpose of the program is to determine the function of nucleic acid sequences but it also offers the simpler listing, searching and counting options. It contains methods to locate genes by looking for the effe...

journal_title：Nucleic acids research

authors： Staden R

更新日期：1984-01-11 00:00:00

abstract：:We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction with the synthetic D...

journal_title：Nucleic acids research

authors： Osinga KA,Van der Bliek AM,Van der Horst G,Groot Koerkamp MJ,Tabak HF,Veeneman GH,Van Boom JH

更新日期：1983-12-20 00:00:00

abstract：:We have sequenced mouse tRNA genes from two recombinant lambda phage. An 1800 bp sequence from one phage contains 3 tRNA genes, potentially encoding tRNAAsp, tRNAGly, and tRNAGlu, separated by spacer sequences of 587 bp and 436 bp, respectively. The mouse tRNA gene cluster is homologous to a rat sequence (Sekiya et al...

journal_title：Nucleic acids research

authors： Looney JE,Harding JD

更新日期：1983-12-20 00:00:00

abstract：:The intragenic control regions of a eukaryotic tRNA gene have been examined by transcribing mutant forms of a Drosophila tRNAArg gene either by injection into the nucleus of Xenopus oocytes or in extracts prepared from isolated oocyte nuclei. These experiments demonstrate that the selection of the transcription initia...

journal_title：Nucleic acids research

authors： Sharp S,Dingermann T,Schaack J,Sharp JA,Burke DJ,DeRobertis EM,Söll D

更新日期：1983-12-20 00:00:00

abstract：:The nucleotide sequence of the chick beta-actin gene was determined. The gene contains 5 introns; 4 interrupt the translated region at codons 41/42, 120/122, 267, 327/328 and a large intron occurs in the 5' untranslated region. The gene has a 97 nucleotide 5'-untranslated region and a 594 nucleotide 3'-untranslated re...

journal_title：Nucleic acids research

authors： Kost TA,Theodorakis N,Hughes SH

更新日期：1983-12-10 00:00:00

abstract：:The cancer drug daunomycin is an effective inhibitor of the B leads to Z transition in poly d(G-C) in 4 M NaCl. Both the rate and extent of the B leads to Z transition are decreased by the drug, as judged by equilibrium and kinetic studies. Daunomycin can, under some conditions, convert Z form DNA back to B form. Drug...

journal_title：Nucleic acids research

authors： Chaires JB

更新日期：1983-12-10 00:00:00

abstract：:We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that...

journal_title：Nucleic acids research

authors： Colantuoni V,Romano V,Bensi G,Santoro C,Costanzo F,Raugei G,Cortese R

更新日期：1983-11-25 00:00:00

abstract：:The replication origin (oriC) of the Escherichia coli chromosome has been cloned and the region essential for chromosomal replication has been delimited to 245 base pairs. In previous studies the ability of recombinants between oriC and ColE1-type vectors, to transform E. coli polA- strains was used to determine which...

journal_title：Nucleic acids research

authors： Stuitje AR,Meijer M

更新日期：1983-11-25 00:00:00

abstract：:The nucleotide sequence of the 5S ribosomal RNA of Streptococcus cremoris has been determined. The sequence is 5' (sequence in text) 3'. Comparison of the S. cremoris 5S RNA sequence to an updated prokaryotic generalized 5S RNA structural model shows that this 5S RNA contains some unusual structural features. These fe...

journal_title：Nucleic acids research

authors： Neimark H,Andersen J,Delihas N

更新日期：1983-11-11 00:00:00

abstract：:In the poly(A)+RNA isolated from the ciliate Paramecium primaurelia is found a discrete and abundant mRNA species of high molecular weight (corresponding to about 9,000 nucleotides). This mRNA species has size and abundance characteristics that identify it tentatively as the message coding for the variant cell-surface...

journal_title：Nucleic acids research

authors： Sommerville J

更新日期：1983-11-11 00:00:00

abstract：:A mutant at the carboxyl end of the terminal protein, p3, of phage phi 29 DNA has been constructed by inserting an containing the stop translation codon TGA in the three possible reading frames, immediately downstream of a phage phi 29 DNA fragment coding for all but the last five amino acids of protein p3. The activ...

journal_title：Nucleic acids research

authors： Mellado RP,Salas M

更新日期：1983-11-11 00:00:00

abstract：:The DNA sequence of the spacer (plus flanking) regions separating the 16S rRNA and 23S rRNA genes of two presumptive rDNA operons of the archaebacterium Methanococcus vannielii was determined. The spacers are 156 and 242 base pairs in size and they share a sequence homology of 49 base pairs following the 3' terminus o...

journal_title：Nucleic acids research

authors： Jarsch M,Böck A

更新日期：1983-11-11 00:00:00

abstract：:Fully protected diastereoisomers of deoxyguanylyl (3' leads to 5') deoxyadenosine stereospecifically labelled on phosphorus with oxygen-18 have been synthesized by oxidation of phosphite triester intermediates in the presence of 18O-labelled water. The diastereoisomers have been chromatographically separated and their...

journal_title：Nucleic acids research

authors： Potter BV,Eckstein F,Uznański B

更新日期：1983-10-25 00:00:00

abstract：:Highly repetitive Hind III restriction fragments of 0.72-0.76 KBP from total Xenopus laevis genomic DNA are organized in a tandem like arrangement. Cloning of these fragments in pBR 322 with subsequent restriction site mapping and nucleotide sequence analysis of some selected clones showed two different types of seque...

journal_title：Nucleic acids research

authors： Meyerhof W,Tappeser B,Korge E,Knöchel W

更新日期：1983-10-25 00:00:00

abstract：:We have determined the nucleotide sequences of long terminal repeat (LTR) regions of Syrian hamster intracisternal A particle (IAP) genes. The size of the LTRs was 350 base-pairs (bp) and 376 bp in two clones, H10 and H18, respectively. Two LTRs at both ends of the IAP gene were linked to directly repeating 6 bp hamst...

journal_title：Nucleic acids research

authors： Ono M,Ohishi H

更新日期：1983-10-25 00:00:00

abstract：:Lytic infection with polyoma virus leads in Go-arrested primary mouse kidney cell cultures to a mitotic host response. In the present work we focused our attention on cellular RNA synthesis shortly after onset of polyoma T-antigen synthesis. Onset of polyoma-induced stimulation of 45S pre-rRNA synthesis was determined...

journal_title：Nucleic acids research

authors： Matter JM,Tiercy JM,Weil R

更新日期：1983-10-11 00:00:00

abstract：:The structures of the termini and their flanking regions of two human KpnI family members were investigated. The two differed in length, but the starting sequence at one terminal (defined as the 5' terminal) was found to be common to both members. The Alu family sequence was found in the 5' flanking regions. The KpnI ...

journal_title：Nucleic acids research

authors： Miyake T,Migita K,Sakaki Y

更新日期：1983-10-11 00:00:00

abstract：:We have constructed and tested several new vectors for P element-mediated gene transfer. These vectors contain restriction sites for cloning a wide variety of DNA fragments within a small, non-autonomous P element and can be used to efficiently transduce microinjected DNA sequences into the germ line chromosomes of D....

journal_title：Nucleic acids research

authors： Rubin GM,Spradling AC

更新日期：1983-09-24 00:00:00

abstract：:The nucleotide sequence for human argininosuccinate synthetase cDNA was determined by analysis of six clones isolated from a single experiment. The sequence covered 1623 nucleotides including 76 bases of poly(A) and contained a 1236 nucleotide open reading frame encoding a protein of 46,434 daltons. In one cDNA isolat...

journal_title：Nucleic acids research

authors： Bock HG,Su TS,O'Brien WE,Beaudet AL

更新日期：1983-09-24 00:00:00

abstract：:A sixty base pair DNA duplex containing the nucleotide sequence of the bacteriophage T5 early (T5P25) promoter has been constructed using a combination of chemical synthesis and enzymatic methods. Subsequent to cloning into pBR322, the promoter has been demonstrated to be biologically active being capable of directing...

journal_title：Nucleic acids research

authors： Rommens J,MacKnight D,Pomeroy-Cloney L,Jay E

更新日期：1983-09-10 00:00:00

abstract：:A type II restriction endonuclease, DraI, isolated from Deinococcus radiophilus ATCC 27603 recognises the palindromic hexanucleotide sequence (formula; see text) and cleaves it, as indicated by the arrows, to produce blunt-ended fragments. The yield of enzyme is 100 to 1000 times that of the only other known type II r...

journal_title：Nucleic acids research

authors： Purvis IJ,Moseley BE

更新日期：1983-08-25 00:00:00

abstract：:We describe a method for the formation of hybrid genes by in vivo recombination between two genes with partial sequence homology. DNA structures consisting of plasmid vector sequences, flanked by the alpha 2 interferon gene on the one side and a portion of the alpha 1 interferon gene (homology about 80%) on the other,...

journal_title：Nucleic acids research

authors： Weber H,Weissmann C

更新日期：1983-08-25 00:00:00

abstract：:DNase I and MPE.Fe (II) footprinting both employ partial cleavage of ligand-protected DNA restriction fragments and Maxam-Gilbert sequencing gel methods of analysis. One method utilizes the enzyme, DNase I, as the DNA cleaving agent while the other employs the synthetic molecule, methidium-propyl-EDTA (MPE). For actin...

journal_title：Nucleic acids research

authors： Van Dyke MW,Dervan PB

更新日期：1983-08-25 00:00:00

abstract：:We have isolated and sequenced the complete murine I-E alpha immune response gene of the H-2db haplotype. The I-E alpha d gene consists of 5300 basepairs and is organized into five or possibly six exons that correspond to different domains of the alpha chain. The amino acid sequence deduced from the I-E alpha gene sho...

journal_title：Nucleic acids research

authors： Hyldig-Nielsen JJ,Schenning L,Hammerling U,Widmark E,Heldin E,Lind P,Servenius B,Lund T,Flavell R,Lee JS,Trowsdale J,Schreier PH,Zablitzky F,Larhammar D,Peterson PA,Rask L

更新日期：1983-08-11 00:00:00

abstract：:We have synthesized the tetradecamer GAGCXGATAACAAG containing a part of the sequence of the lactose operator. A guanine base in the sequence is replaced by the adduct of the carcinogen 2-acetylaminofluorene with guanine. Under the standard conditions of de-protection, the fluorene moiety is lost, leaving behind a gua...

journal_title：Nucleic acids research

authors： Stöhrer G,Osband JA,Alvarado-Urbina G

更新日期：1983-08-11 00:00:00

abstract：:Protein species interacting with newly replicated DNA were analyzed using a photo cross-linking technique. Nascent DNA was labeled in vitro with [alpha-32P]dCTP and BrdUTP in SV40-infected CV-1 cells made permeable with saponin. The labeled cells were then irradiated with UV light (254 nm) and were treated extensively...

journal_title：Nucleic acids research

authors： Tsutsui K,Watanabe S,Katagiri M,Oda T

更新日期：1983-07-25 00:00:00

abstract：:To determine whether methylation changes in specific DNA sequences of the albumin and AFP genes are implicated in the modulation of transcriptional activity during rat liver development and neoplasia we have analysed the methylation pattern of C-C-G-G sequences within these genes in DNA isolated from fetal and adult h...

journal_title：Nucleic acids research

authors： Vedel M,Gomez-Garcia M,Sala M,Sala-Trepat JM

更新日期：1983-07-11 00:00:00

abstract：:The hexanucleoside pentaphosphate d(m5CpGpm5CpGpm5CpG) has been studied in solution by ultra-violet absorption, circular dichroism and 31P nuclear magnetic resonance under various experimental conditions. In 0.2 M NaClO4 at low temperature, an hexamer duplex is formed which has a B or B-like conformation. As the salt ...

journal_title：Nucleic acids research

authors： Hartmann B,Thuong NT,Pouyet J,Ptak M,Leng M

更新日期：1983-07-11 00:00:00

abstract：:A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formu...

journal_title：Nucleic acids research

authors： Seurinck J,Van de Voorde A,Van Montagu M

更新日期：1983-07-11 00:00:00

abstract：:We have microinjected genomic DNA clones containing the Moloney murine leukemia virus (M-MuLV) proviral genome and flanking mouse sequences from Mov-3, Mov-7 and Mov-10 mice into Xenopus laevis oocytes and analyzed the virus-specific transcription and translation products. These mouse strains carry a proviral genome c...

journal_title：Nucleic acids research

authors： Breindl M,Kalthoff H,Jaenisch R

更新日期：1983-06-25 00:00:00

abstract：:An open reading frame upstream from nifHDK operon of Klebsiella pneumoniae had been described. The orientation of this open reading frame is opposite to that of nifHDK and sequence homology was found between the open reading frame promoter and the promoter of nifHDK operon. A recombinant plasmid carrying the promoter ...

journal_title：Nucleic acids research

authors： Shen SC,Xue ZT,Kong QI,Wu QL

更新日期：1983-06-25 00:00:00

abstract：:It has been recently reported that the pR plasmid enhances the UV survival in E.coli c600. In order to test whether this function may be expressed also in mammalian cells, LTA (tk- aprt-) mouse cells were cotransformed with pR plasmid DNA and ptk1 plasmid as selectable marker. Tk+ transformants were analyzed for their...

journal_title：Nucleic acids research

authors： Elli R,Marcucci L,Bosi R,Oppi C,Battaglia PA,Gigliani F

更新日期：1983-06-11 00:00:00

abstract：:Two hexamer DNA fragments containing a carcinogenic modified base, O6-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were O6 protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's...

journal_title：Nucleic acids research

authors： Kuzmich S,Marky LA,Jones RA

更新日期：1983-05-25 00:00:00

abstract：:Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metallothionein (MT) gene in adult mouse liver, when induced to produce mRNA by injection of cadmium, becomes more susceptible to nuc...

journal_title：Nucleic acids research

authors： Koropatnick J,Andrews G,Duerksen JD,Varshney U,Gedamu L

更新日期：1983-05-25 00:00:00

abstract：:Equilibrium and kinetics of thermal melting of yeast 5S ribosomal RNA in aqueous NaCl with or without Mg2+ were investigated by differential thermal melting and temperature jump methods. Two peaks (1 and 2) and a shoulder were observed in each of melting curves at ionic strength I=0.002-0.5 and linearity between each ...

journal_title：Nucleic acids research

authors： Ohta S,Maruyama S,Nitta K,Sugai S

更新日期：1983-05-25 00:00:00

abstract：:A gene bank of Staphylococcus aureus strain Cowan I was established using an E. coli HB101/pBR327 host-vector system. Recombinants expressing staphylococcal protein A (SPA) were detected using an IgG-binding assay. A 3.2 Kb DNA fragment directing the synthesis of SPA in E. coli was identified. SPA produced by E. coli ...

journal_title：Nucleic acids research

authors： Duggleby CJ,Jones SA

更新日期：1983-05-25 00:00:00

abstract：:We have determined the complete nucleotide sequence of the regions of mouse ribosomal RNA transcription unit which separate mature rRNA genes. These internal transcribed spacers (ITS) are excised from rRNA precursor during ribosome biosynthesis. ITS 1, between 18S and 5.8S rRNA genes, is 999 nucleotides long. ITS 2, b...

journal_title：Nucleic acids research

authors： Michot B,Bachellerie JP,Raynal F

更新日期：1983-05-25 00:00:00

abstract：:The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reac...

journal_title：Nucleic acids research

authors： Dörper T,Winnacker EL

更新日期：1983-05-11 00:00:00

abstract：:A single-helical B-type geometry is presented based on 1H NMR observations on d(TATA) and several other small single-helical DNA fragments. The structure is extended to one complete turn of double-helical DNA and its characteristics are compared with other known B-type structures. ...

journal_title：Nucleic acids research

authors： Mellema JR,van Kampen PN,Carlson CN,Bosshard HE,Altona C

更新日期：1983-05-11 00:00:00